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Aνακοινώθηκε στο 11th World Congress of the International Association of

Surgeons & Gastrenterologists (Former International Gastro- Surgical Club – ICSC)
Heraklion, Crete – Greece 1-4 Nov 2001

Δημοσιεύτηκε η περίληψη στο περιοδικό Hepatogastroenterology 48 (Suppl. I):11,

2001

Abstract

Background. In Intensive Care Units, 100-fold doses compared to daily recommend

ed allowances are used: vit C 3 to 4g and vit Ε l to 2g/IV in slow drip/day, as :&00
radical (FR) scavengers. We tested the hypothesis of supranutritionall00 fold doses

in rat liver Ischemia (l) and Reperfusion injury (R), not previously reported in the
bibliography.

Methods. Thirty fed male Albino WlStar rats weighing 300 Ιο 350 g, were separated

into Group Α:. 10 rats. Νο ischemia or medicine. Group Β: 10 rats; subjected to 90
min Ι and 60 min R by clamping of the median and left lobe of the liver (73% rat

model by Jeppson, Sweden). Νο medicine was used. Group C: 10 rats with 90 min Ι
and 60 min R pretreated with ΙΜ vit C 50mg and ΙΜ vit Ε 300mg (d-La-toc 15%

solution La Roche, 8witz) for 3 days and οηε dose prior to surgery (equivalent to
15gC + 70gE in a 70 kg human). Κetamine and Fentanyl ΙΜ, for anesthesia. Liver

Malondialdehyde (MDA) an end product oflipid peroxidation (LP) was measured in all
groups after the R period.

ResuIts. Mean ΜDΑ values (nmol/g wet tissue) were. Group Α (0.771+0.lO2) Group Β

(l.332+0.097) Group C (Ο.694+Ο.163). 8tudent's t-test showed statistical significant
differences between groups Β and Α ρ<Ο.ΟΟl, Β and C ρ<Ο.ΟΟΟ, C and Α ρ<Ο.185.

Liver function tests correlated with liver damage, but afforded no specific value in
titration of LP. Light Histology showed more hepatocytes with intact membranes,

better architectural morphology ίη cells aligning οr near vessels, lesser degree of
destruction ίη group C compared to group Κ Electron microscopy (ΤΕΜ) revealed

more glycogen particles and rough endoplasmic reticulum in group C, than in Β.

Conclusions: 1) 9O min of liver Ι creates a "high level" of destruction compared to
"medium level" 6Omin Ι model commonly used causing LP of cells. 2) Vιt C and Ε

gave better results, improved the antioxidant level as compared to οηθ substance
(single νιt C 2Omg showed ηο effect, used by us ίη the past). 3) Diffusion of vitamins

to cells located in anatomical liver acinar Zones Ι and II seems to provide better

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